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fluorescein conjugated avidin  (Vector Laboratories)


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    Vector Laboratories fluorescein conjugated avidin
    Fluorescein Conjugated Avidin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 906 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/A-2001-5/Fluorescein+Avidin+D/pm42867378-54-29-32
    Average 94 stars, based on 906 article reviews
    fluorescein conjugated avidin - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Hybridization:

    Article Title: Development and Characterization of Wheat- Thinopyrum elongatum 1B-1E Translocation Lines with Fusarium Head Blight Resistance
    Article Snippet: .. The Th. elongatum E genome probe and oligo pSc119.2-1 applied simultaneously under the hybridization condition of 69 °C for 2 min. Hybridization signals of Oligo-p119.2-1 was detected with fluorescein isothiocyanate-conjugated avidin (FITC-avidin) (Vector Laboratories, Inc., Newark, CA, USA) as green. .. Hybridization signals on Th. elongatum chromatin and Oligo-pTa535-1 were detected with Anti-Digoxigenin-Rhodamine (Roche, REF: 11207750910) as red, and wheat chromatin was counter-stained with DAPI (Roche, REF : 10236276001) as blue.

    Avidin-Biotin Assay:

    Article Title: Development and Characterization of Wheat- Thinopyrum elongatum 1B-1E Translocation Lines with Fusarium Head Blight Resistance
    Article Snippet: .. The Th. elongatum E genome probe and oligo pSc119.2-1 applied simultaneously under the hybridization condition of 69 °C for 2 min. Hybridization signals of Oligo-p119.2-1 was detected with fluorescein isothiocyanate-conjugated avidin (FITC-avidin) (Vector Laboratories, Inc., Newark, CA, USA) as green. .. Hybridization signals on Th. elongatum chromatin and Oligo-pTa535-1 were detected with Anti-Digoxigenin-Rhodamine (Roche, REF: 11207750910) as red, and wheat chromatin was counter-stained with DAPI (Roche, REF : 10236276001) as blue.

    Article Title: Pre-injury subchronic stress confers sex-specific protection against pain-associated symptoms in osteoarthritic mice
    Article Snippet: .. Finally, tissue was incubated for 5min with tyramide solution (Ape Bio, A8011-APE), washed 2 x 15min with 0.1M PB, then incubated for an additional 2h with Avidin D, Fluorescein labelled (Vector Labs, A-2001-5). ..

    Article Title: Germline-Restricted Chromosome (GRC) in Diploid and Polyploid Spermatocytes of the Eurasian Bullfinch, Pyrrhula pyrrhula (Fringillidae, Passeriformes, Aves).
    Article Snippet: .. Biotin-labeled GRC probe was detected using avidin-FITC (Vector Laboratories, Newark, CA, USA; catalog no. A-2001-5) and anti-avidin-FITC antibodies (Vector Laboratories, Newark, CA, USA). .. Slides were mounted in Vectashield medium with DAPI (Vector Laboratories, Newark, CA, USA; catalog no. H-1200-10).

    Article Title: Voluntary wheel running provides pain relief but transiently exacerbates gait impairments in male and female mice with unilateral osteoarthritis
    Article Snippet: Next day, sections were washed in 0.1M PB then incubated for 2h with rabbit biotinylated secondary antibody (1:400; Bethyl Labs, A120-108B), followed by incubation in preformed avidin-biotin-HRP complexes (1:200; Vectastain ABCKit Peroxidase, PK-6100) for 30min. .. Finally, tissue was incubated for 5min with tyramide solution (ApeBio, A8011-APE), washed 2×15min with 0.1M PB, then incubated for an additional 2h with Avidin D, Fluorescein labelled (Vector Labs, A-2001-5). .. Sections were given a final wash, mounted on gelatinised slides, and coverslipped with Fluoromount Aqueous Mounting Medium (Sigma-Aldrich, F4680).

    Article Title: First cytogenetic characterization of the southern pygmy perch Nannoperca australis (Teleostei: Percichthyidae): Karyotype, heterochromatin and repetitive DNA organization.
    Article Snippet: The probe-chromosome hybridization was performed overnight at 37 C. Post-hybridization washes were performed as follows: three washes for 5 min in 50% formamide/2 SSC at 37 C, two washes for 5 min in 2 SSC and one final wash for 5 min in 4 SSC/0.05% Tween 20 (Sigma Aldrich). .. Slides were first incubated for 30 min at 37 C with 4 SSC/5% blocking reagent (Roche), then the probe signal was detected by 4 SSC/5% blocking reagent mixed with fluorescein conjugated avidin (Vector laboratories) for 30 min at 37 C, followed by three washes in 4 SSC/0.05% Tween 20 for 5 min. .. Slides were then incubated with biotinylated anti-avidin (Vector laboratories) for 30 min at 37 C, followed by a second round of fluorescein conjugated avidin treatment for signal amplification.

    Article Title: Molecular cytogenetic analysis of some Picea species from Eurasia and East Asia
    Article Snippet: For this purpose, the cloned rDNA PCR product was labelled with Biotin-16-dUTP (Roche, Germany) by nick translation, according to the manufacturer’s protocol. .. The biotinylated probes were detected using avidin, conjugated with fluorescein (Fluorescein Avidin D, Vector Laboratories, USA). .. The hybridization signal was enhanced using fluorescein anti-avidin (Fluorescein Anti-Avidin D, Vector Laboratories, USA).

    Article Title: Transient silencing of spinal nociceptive neurons leads to a long-term reduction in joint pain mediated by recruitment of opioid activity
    Article Snippet: Then, sections were incubated with avidin-biotin complex in PBS-T (1:250 Vectastain A plus and 1:250 Vectastain B; ABC Kit Peroxidase Standard, Vector Laboratories) for 30 minutes at room temperature, followed by a signal amplification step with biotinylated tyramide solution (1:75 for 5 minutes; Startech). .. Finally, sections were incubated with Fluorescein Avidin D (1:600, Vector Laboratories) for 2 hours at room temperature. ..

    Incubation:

    Article Title: Calretinin-Positive Neurons in Central Medial Thalamus Drive Nociception and Anxiety via Projections to Anterior Insular Cortex in male mice
    Article Snippet: .. The sections were 249 incubated with FITC-Avidin (1:500, A-2001, Vectorlabs), Alexa Fluor 594-conjugated 250 donkey anti-mouse (1:500, ab150112, Abcam) and Alexa Fluor 647-conjugated donkey 251 anti-rabbit (1:500, ab150075, Abcam) in TBS (0.1 M Tris-HCl, 0.9% saline) for 4 h. 252 Finally, the sections were washed in TNT three times, each for 10 min. 253 2.7 Behavior assays 254 All experiments were conducted during the light cycle (8 a.m. to 8 p.m.). ..

    Article Title: Pre-injury subchronic stress confers sex-specific protection against pain-associated symptoms in osteoarthritic mice
    Article Snippet: .. Finally, tissue was incubated for 5min with tyramide solution (Ape Bio, A8011-APE), washed 2 x 15min with 0.1M PB, then incubated for an additional 2h with Avidin D, Fluorescein labelled (Vector Labs, A-2001-5). ..

    Article Title: Voluntary wheel running provides pain relief but transiently exacerbates gait impairments in male and female mice with unilateral osteoarthritis
    Article Snippet: Next day, sections were washed in 0.1M PB then incubated for 2h with rabbit biotinylated secondary antibody (1:400; Bethyl Labs, A120-108B), followed by incubation in preformed avidin-biotin-HRP complexes (1:200; Vectastain ABCKit Peroxidase, PK-6100) for 30min. .. Finally, tissue was incubated for 5min with tyramide solution (ApeBio, A8011-APE), washed 2×15min with 0.1M PB, then incubated for an additional 2h with Avidin D, Fluorescein labelled (Vector Labs, A-2001-5). .. Sections were given a final wash, mounted on gelatinised slides, and coverslipped with Fluoromount Aqueous Mounting Medium (Sigma-Aldrich, F4680).

    Article Title: First cytogenetic characterization of the southern pygmy perch Nannoperca australis (Teleostei: Percichthyidae): Karyotype, heterochromatin and repetitive DNA organization.
    Article Snippet: The probe-chromosome hybridization was performed overnight at 37 C. Post-hybridization washes were performed as follows: three washes for 5 min in 50% formamide/2 SSC at 37 C, two washes for 5 min in 2 SSC and one final wash for 5 min in 4 SSC/0.05% Tween 20 (Sigma Aldrich). .. Slides were first incubated for 30 min at 37 C with 4 SSC/5% blocking reagent (Roche), then the probe signal was detected by 4 SSC/5% blocking reagent mixed with fluorescein conjugated avidin (Vector laboratories) for 30 min at 37 C, followed by three washes in 4 SSC/0.05% Tween 20 for 5 min. .. Slides were then incubated with biotinylated anti-avidin (Vector laboratories) for 30 min at 37 C, followed by a second round of fluorescein conjugated avidin treatment for signal amplification.

    Article Title: Transient silencing of spinal nociceptive neurons leads to a long-term reduction in joint pain mediated by recruitment of opioid activity
    Article Snippet: Then, sections were incubated with avidin-biotin complex in PBS-T (1:250 Vectastain A plus and 1:250 Vectastain B; ABC Kit Peroxidase Standard, Vector Laboratories) for 30 minutes at room temperature, followed by a signal amplification step with biotinylated tyramide solution (1:75 for 5 minutes; Startech). .. Finally, sections were incubated with Fluorescein Avidin D (1:600, Vector Laboratories) for 2 hours at room temperature. ..

    Transcranial Magnetic Stimulation:

    Article Title: Calretinin-Positive Neurons in Central Medial Thalamus Drive Nociception and Anxiety via Projections to Anterior Insular Cortex in male mice
    Article Snippet: .. The sections were 249 incubated with FITC-Avidin (1:500, A-2001, Vectorlabs), Alexa Fluor 594-conjugated 250 donkey anti-mouse (1:500, ab150112, Abcam) and Alexa Fluor 647-conjugated donkey 251 anti-rabbit (1:500, ab150075, Abcam) in TBS (0.1 M Tris-HCl, 0.9% saline) for 4 h. 252 Finally, the sections were washed in TNT three times, each for 10 min. 253 2.7 Behavior assays 254 All experiments were conducted during the light cycle (8 a.m. to 8 p.m.). ..

    Saline:

    Article Title: Calretinin-Positive Neurons in Central Medial Thalamus Drive Nociception and Anxiety via Projections to Anterior Insular Cortex in male mice
    Article Snippet: .. The sections were 249 incubated with FITC-Avidin (1:500, A-2001, Vectorlabs), Alexa Fluor 594-conjugated 250 donkey anti-mouse (1:500, ab150112, Abcam) and Alexa Fluor 647-conjugated donkey 251 anti-rabbit (1:500, ab150075, Abcam) in TBS (0.1 M Tris-HCl, 0.9% saline) for 4 h. 252 Finally, the sections were washed in TNT three times, each for 10 min. 253 2.7 Behavior assays 254 All experiments were conducted during the light cycle (8 a.m. to 8 p.m.). ..

    Blocking Assay:

    Article Title: First cytogenetic characterization of the southern pygmy perch Nannoperca australis (Teleostei: Percichthyidae): Karyotype, heterochromatin and repetitive DNA organization.
    Article Snippet: The probe-chromosome hybridization was performed overnight at 37 C. Post-hybridization washes were performed as follows: three washes for 5 min in 50% formamide/2 SSC at 37 C, two washes for 5 min in 2 SSC and one final wash for 5 min in 4 SSC/0.05% Tween 20 (Sigma Aldrich). .. Slides were first incubated for 30 min at 37 C with 4 SSC/5% blocking reagent (Roche), then the probe signal was detected by 4 SSC/5% blocking reagent mixed with fluorescein conjugated avidin (Vector laboratories) for 30 min at 37 C, followed by three washes in 4 SSC/0.05% Tween 20 for 5 min. .. Slides were then incubated with biotinylated anti-avidin (Vector laboratories) for 30 min at 37 C, followed by a second round of fluorescein conjugated avidin treatment for signal amplification.



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